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Anti -EGFP Rabbit pAb

價格: 在線咨詢
貨號:
DRF10129
產(chǎn)品規(guī)格:
100 μL
Applications:
WB | IF
Species:
--
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

EGFP rabbit polyclonal

Protein full name

Enhanced GFP

Synonyms

Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP

Immunogen

Recombinant protein corresponding to EGFP( enhanced green fluorescent protein)

Isotype

IgG

Purity

Affinity purification

Predicted MW.

27 kDa

Observed MW.

29 kDa

Uniprot ID

P42212




Applications


Applications

Species

Dilution

Positive Tissue

WB

/

1: 500-1: 1000

cells/ tissues with eGFP 

IF

/

1: 500-1: 1000

293T



Background


Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining. Other applications of eGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.




Images



圖片 6.png

Western blot analysis of eGFP (GB11602) 

at dilution of 1: 500

Lane 1: 293T cell lysate

Lane 2: 293T with eGFP cell lysate

Lane 3: NIH/3T3 cell lysate

Lane 4: NIH/3T3 with eGFP cell lysate

image.png

Immunofluorescence analysis of human 293T cells using eGFP (GB11602) at dilution of 1: 1000

A: DAPI nuclear stain ( blue )

B: eGFP protein ( green )

C: Anti-eGFP ( red )

D: Merged




Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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