Anti -EGFP Rabbit pAb

價格：在線咨詢

貨號：

DRF10129

產(chǎn)品規(guī)格：

100 μL

Applications：

WB | IF

Species：

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項

Product Information

Description	EGFP rabbit polyclonal
Protein full name	Enhanced GFP
Synonyms	Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP
Immunogen	Recombinant protein corresponding to EGFP( enhanced green fluorescent protein)
Isotype	IgG
Purity	Affinity purification
Predicted MW.	27 kDa
Observed MW.	29 kDa
Uniprot ID	P42212

Applications

Applications	Species	Dilution	Positive Tissue
WB	/	1: 500-1: 1000	cells/ tissues with eGFP
IF	/	1: 500-1: 1000	293T

Background

Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining. Other applications of eGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols.

Images

Western blot analysis of eGFP (GB11602)

at dilution of 1: 500

Lane 1: 293T cell lysate

Lane 2: 293T with eGFP cell lysate

Lane 3: NIH/3T3 cell lysate

Lane 4: NIH/3T3 with eGFP cell lysate

Immunofluorescence analysis of human 293T cells using eGFP (GB11602) at dilution of 1: 1000

A: DAPI nuclear stain ( blue )

B: eGFP protein ( green )

C: Anti-eGFP ( red )

D: Merged

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer	PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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