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Anti -TNF-α Rabbit pAb

價格: 在線咨詢
貨號:
DRF101674
產(chǎn)品規(guī)格:
100 μL
Applications:
Species:
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

TNF-α rabbit polyclonal

Protein full name

Tumor necrosis factor alpha

Synonyms

TNF,DIF,TNF-alpha,TNFA,TNFSF2,Tumour necrosis factor, Tumour necrosis factor alpha, TNF-α, tumor necrosis factor, TNLG1

Immunogen

KLH conjugated Synthetic peptide

corresponding to Mouse TNF-α

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cell membrane, Secreted

Predicted MW.

26 kDa

Observed MW.

26 kDa

Uniprot ID

P01375, P06804



Applications


Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse, Rat

1: 200-1: 500

lymph node, thymus, spleen, lung

IHC

Mouse

1: 400-1: 1000

lung, septic lung, hepatitis

IF

Mouse

1: 300-1: 800

lung 



Background


Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.




Images


Western blot analysis of TNF-α (GB11188) 

at dilution of 1: 500

Western blot analysis of TNF-α (GB11188) 

at dilution of 1: 500

Immunohistochemistry analysis of paraffin-embedded mouse lung using 

TNF-α(GB11188)at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded mouse septic lung using 

TNF-α(GB11188)at dilution of 1: 1000

Immunohistochemistry analysis of paraffin-embedded mouse hepatitis using 

TNF-α(GB11188)at dilution of 1: 1000

Immunofluorescent analysis of paraformaldehyde-fixed mouse lung using 

TNF-α(GB11188)at dilution of 1: 400




Storage


Storage Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .


Publications:



Title

Journal

Pubmed ID

Application

1

Early Enteral Nutrition Preserves Intestinal Barrier Function through Reducing the Formation of Neutrophil Extracellular Traps (NETs) in Critically Ill Surgical Patients.

Oxidative Medicine and Cellular Longevity

33294125

IHC

2

QKI deficiency leads to osteoporosis by promoting RANKL-induced osteoclastogenesis and disrupting bone metabolism.

Cell Death & Disease

32382069

IHC

3

Liver-target nanotechnology facilitates berberine to ameliorate cardio-metabolic diseases.

Nature Communications

31040273

WB

4

Induction of pathological changes and impaired expression of cytokines in developing female rat spleen after chronic excess fluoride exposure.

Toxicology and Industrial Health

30463494

IHC

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