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Anti -GFAP Rabbit pAb

價(jià)格: 在線咨詢(xún)
貨號(hào):
KT011563
產(chǎn)品規(guī)格:
100 μL
Applications:
WB | IHC | IF
Species:
H,M,R/M.R
購(gòu)買(mǎi)數(shù)量:
產(chǎn)品詳情 操作說(shuō)明 注意事項(xiàng)

Product Information


Description

GFAP rabbit polyclonal antibody

Protein full name

Glial fibrillary acidic protein

Synonyms

GFAP, ALXDRD, glial fibrillary acidic protein, Astrocyte, Intermediate filament protein

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse GFAP

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

49 kDa

Observed MW.

49 kDa

Uniprot ID

P14136, P03995, P47819



Applications


Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 1000-1: 2000

brain, cerebellum, cortex

IHC/IF

Mouse, Rat

1: 500-1: 3000

brain, cerebellum



Background


Glial fibrillary acidic protein is an intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS) including astrocytes and ependymal cells.GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. It is closely related to its non-epithelial family members, vimentin,desmin, and peripherin, which are all involved in the structure and function of the cell’s cytoskeleton.




Images



Western blot analysis of GFAP (GB11096) at dilution of 1: 1000

Immunohistochemistry of paraffin embedded rat brain using GFAP (GB11096) at dilution of 1: 3000 (200x lens)

Immunohistochemistry of paraffin embedded mouse brain using GFAP (GB11096) at dilution of 1: 3000 (400x lens)

Immunohistochemistry of paraffin embedded rat cerebellum using GFAP (GB11096) at dilution of 1: 2800 (200x lens)


Immunofluorescence of paraffin embedded mouse brain using GFAP (GB11096) at dilution of 1: 2000 (400x lens)


Immunofluorescence of paraffin embedded rat brain using GFAP (GB11096) at dilution of 1: 2000 (400x lens)





Storage



Storage Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer.

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