160 kDa Neurofilament Medium mouse monoclonal antibody
Protein full name
Neurofilament medium polypeptide
Synonyms
160 kDa neurofilament protein, Neurofilament 3, Neurofilament triplet M protein, Nef3, Nefm, NF-M, Nfm, 150kDa medium
Immunogen
Recombinant protein corresponding to Mouse NF-M
Isotype
IgG
Purity
Affinity purification
Subcellular location
Cytoplasm, Cytoskeleton
Predicted MW.
96 kDa
Observed MW.
150 kDa
Uniprot ID
P07197, P08553, P12839
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse, Rat
1: 500-1: 1000
brain, cerebral cortex, hippocampus, spinal cord
IHC/IF
Mouse, Rat
1: 500-1: 4000
brain, spinal cord, epencephalon
Background
NEFM, also named as NEF3 and NFM, belongs to the intermediate filament family. Neurofilaments are the 10nm intermediate filaments found specifically in neurons. They are a major component of the cell's cytoskeleton, and provide support for normal axonal radial growth. Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.
Images
Western blot analysis of NF-M (GB12763) at dilution of 1: 1000
Immunohistochemistry analysis of paraffin-embedded mouse brain using NF-M (GB12763) at dilution of 1: 4000
Immunohistochemistry analysis of paraffin-embedded rat brain using NF-M (GB12763) at dilution of 1: 4000
Immunohistochemistry analysis of paraffin-embedded mouse spinal cord using NF-M (GB12763) at dilution of 1: 4000
Immunohistochemistry analysis of paraffin-embedded rat spinal cord using NF-M (GB12763) at dilution of 1: 4000
Immunohistochemistry analysis of paraffin-embedded mouse epencephalon using NF-M (GB12763) at dilution of 1: 4000
Immunohistochemistry analysis of paraffin-embedded rat epencephalon using NF-M (GB12763) at dilution of 1: 4000
Immunofluorescent analysis of paraformaldehyde-fixed mouse brain using NF-M (GB12763) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed rat brain using NF-M (GB12763) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed mouse spinal cord using NF-M (GB12763) at dilution of 1: 1000
Immunofluorescent analysis of paraformaldehyde-fixed rat spinal cord using NF-M (GB12763) at dilution of 1: 1000
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .