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Anti -EGFP Mouse mAb
價格：
在線咨詢
貨號：
DRF10134
產(chǎn)品規(guī)格：
100 μL
Applications：
WB | IF
Species：
tag/tag
購買數(shù)量：
產(chǎn)品詳情
操作說明
注意事項
Product Information
|
Description |
EGFP mouse monoclonal |
|
Protein full name |
Enhanced GFP |
|
Synonyms |
Enhanced Green Fluorecent protein, rEGFP, Recombinant EGFP, Enhanced Green Fluorecent protein, eGFP, EGFP |
|
Immunogen |
Recombinant protein corresponding to Mouse EGFP |
|
Isotype |
IgG |
|
Purity |
Affinity purification |
|
Predicted MW. |
27 kDa |
|
Observed MW. |
29 kDa |
Applications
|
Applications |
Species |
Dilution |
Positive Tissue |
|
WB |
tag |
1: 500-1: 1000 |
cells/ tissues with eGFP |
|
IF |
tag |
1: 2000-1: 4000 |
CHO-S |
Background
Enhanced green fluorescence protein (eGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). eGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. eGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize eGFP-tagged proteins within living cells without the need for chemical staining.
Images
|
Western blot analysis of eGFP (GB12602) at dilution of 1: 1000 Lane 1: 293T cell lysate Lane 2: 293T with eGFP cell lysate Lane 3: NIH/3T3 cell lysate Lane 4: NIH/3T3 with eGFP cell lysate |
|
|
 B
|
|
Immunofluorescent analysis of transfect CHO-S cells using eGFP (GB12602) at dilution of 1: 2000
A: DAPI nuclear stain ( blue ) B: Anti-eGFP ( red ） |
|
Storage
| Storage | Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .
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合肥萬物生物科技有限公司
客服熱線：400-1016-218
QQ：355185756
地址：安徽省合肥市高新區(qū)黃山路602號合肥國家大學科技園A401室