PABPC1 binds the poly(A) tail of mRNA. It may be involved in cytoplasmic regulatory processes of mRNA metabolism such as pre-mRNA splicing. Its function in translational initiation regulation can either be enhanced by PAIP1 or repressed by PAIP2. PABPC1 can probably bind to cytoplasmic RNA sequences other than poly(A) in vivo. It may be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
Images
Western blot analysis of PABP (GB111164) at dilution of 1: 1000
Lane 1: HT29 cell lysate
Lane 2: Hela, cell lysate
Lane 3: NIH/3T3 cell lysate
Lane 4: Mouse liver tissue lysate
Lane 5: Mouse testis tissue lysate
Lane 6: Mouse brain tissue lysate
Lane 7: Rat liver tissue lysate
Lane 8: Rat testis tissue lysate
Lane 9: Rat brain tissue lysate
Immunohistochemistry analysis of paraffin-embedded mouse testis using PABP (GB111164) at dilution of 1: 1200
Immunohistochemistry analysis of paraffin-embedded rat testis using PABP (GB111164) at dilution of 1: 1200
Immunofluorescent analysis of paraffin-embedded mouse testis using PABP (GB111164) at dilution of 1: 1200
Immunofluorescent analysis of paraffin-embedded rat testis using PABP (GB111164) at dilution of 1: 1200
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/ thaw cycles.
Storage Buffer
PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .