Recombinant protein corresponding to Human Glutamine Synthetase
Isotype
IgG
Purity
Affinity purification
Subcellular location
Cell membrane, Cytoplasm
Predicted MW.
42 kDa
Observed MW.
42 kDa
Uniprot ID
P15104, P15105, P09606
Applications
Applications
Species
Dilution
Positive Sample
WB
Human, Mouse, Rat
1:500-1:1000
liver, testis, brain
IHC/IF
Human, Mouse, Rat
1: 3000-1: 6000
liver, liver cancer, glioma, brain
Background
Glutamine
Synthetase catalyzes the conversion of ammonia and glutamate to
glutamine. It is found in astrocytes as an octamer of identical 42 kDa
subunits. The function of Glutamine Synthetase is the detoxification of
brain ammonia. It also has an important role in the metabolic regulation
of neurotransmitter glutamate. Because of the multiple functions and
importance of Glutamine Synthetase in cellular metabolism, both
catalytic activities and synthesis are highly regulated. The activity of
Glutamine Synthetase is controlled by adenylylation.
Images
Western blot analysis of Glul (GB121177) at dilution of 1: 1000
Immunohistochemistry analysis of paraffin-embedded human liver using GLUL (GB121177) at dilution of 1: 6000
Immunohistochemistry analysis of paraffin-embedded rat liver using
GLUL (GB121177) at dilution of 1: 6000
Immunohistochemistry analysis of paraffin-embedded human liver cancer using GLUL (GB121177) at dilution of 1: 6000
Immunohistochemistry analysis of paraffin-embedded human glioma using
GLUL (GB121177) at dilution of 1: 600
Immunohistochemistry analysis of paraffin-embedded mouse brain using
GLUL (GB121177) at dilution of 1: 6000
Storage
Storage
Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer
PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.
NOTE:
1.This product is intended for research only.
2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .