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Anti -beta Actin Rabbit pAb

價格: 在線咨詢
貨號:
KT011810
產(chǎn)品規(guī)格:
100ul
Applications:
WB/IHC/IF
Species:
H/M/R,H/M/R,H/M/R
購買數(shù)量:
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產(chǎn)品詳情 操作說明 注意事項

Product Information


Description

beta Actin rabbit polyclonal antibody

Protein full name

Actin, cytoplasmic 1

Synonyms

ACTB, BRWS1, PS1TP5BP1, Beta-actin, actin beta, Beta Cytoskeletal Actin

Immunogen

KLH conjugated Synthetic peptide corresponding to Mouse β-Actin

Isotype

IgG

Purity

Affinity purification

Subcellular location

Cytoplasm

Predicted MW.

42 kDa

Observed MW.

45 kDa

Uniprot ID

P60710




Applications



Applications

Species

Dilution

Positive Sample

WB

Human, Mouse, Rat

1: 1000-1: 2000

lung, kidney, brain

IHC/IF

Human, Mouse, Rat

1: 500-1: 1000

colon cancer, liver

ICC/IF

Human, Mouse, Rat

1: 300-1: 1000

NIH/3T3, A549, etc.





Background


Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins coexist in most cell types as components of the cytoskeleton and as mediators of internal cell motility .




Images





Western blot analysis of beta-Actin (GB11001) at dilution of 1: 1000

Immunofluorescence of paraffin embedded human colon cancer using beta-Actin (GB11001) at dilution of 1: 1000


Storage



Storage

Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.

Storage Buffer

PBS with 0.02% sodium azide,100 μg/ml BSA and 50% glycerol.




NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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