Anti -Cyclin B1 Rabbit pAb

價(jià)格：在線咨詢

貨號(hào)：

KT011884

產(chǎn)品規(guī)格：

100 μL

Applications：

WB | IHC | IF

Species：

H,M,R | H,M,R | H,M,R

品牌：

萬物

購買數(shù)量：

立即詢價(jià) 在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項(xiàng)

Product Information

Description	Cyclin B1 rabbit polyclonal
Protein full name	G2/mitotic-specific cyclin-B1
Synonyms	Ccn-2, Ccnb1-rs13, Cycb, Cycb1, Ccnb1, CCNB
Immunogen	KLH conjugated Synthetic peptide corresponding to Mouse Cyclin B1
Isotype	IgG
Purity	Affinity purification
Subcellular location	Cytoplasm, Cytoskeleton, Nucleus
Predicted MW.	48 kDa
Observed MW.	55 kDa
Uniprot ID	P14635, P24860, P30277

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	testis, colon, spleen
IHC	Human, Mouse, Rat	1: 1500-1: 3000	colon cancer, skin, colon
IF	Human, Mouse, Rat	1: 400-1: 800	lung cancer, cervical cancer, testis, colon

Background

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.

Images

	Western blot analysis of Cyclin B1 (GB112098) at dilution of 1: 1000 Lane 1: Jurkat cell lysate Lane 2: 293T cell lysate Lane 3: Mouse testis tissue lysate Lane 4: Mouse colon tissue lysate Lane 5: Rat testis tissue lysate Lane 6: Rat colon tissue lysate Lane 7: Rat spleen tissue lysate
	Immunohistochemistry analysis of paraffin-embedded human colon cancer using Cyclin B1 (GB112098) at dilution of 1: 3000
	Immunohistochemistry analysis of paraffin-embedded human skin using Cyclin B1 (GB112098) at dilution of 1: 3000
	Immunohistochemistry analysis of paraffin-embedded mouse colon using Cyclin B1 (GB112098) at dilution of 1: 3000
	Immunohistochemistry analysis of paraffin-embedded rat colon using Cyclin B1 (GB112098) at dilution of 1: 3000
	Immunofluorescent analysis of paraformaldehyde-fixed human lung cancer using Cyclin B1 (GB112098) at dilution of 1: 800
	Immunofluorescent analysis of paraformaldehyde-fixed human cervical cancer using Cyclin B1 (GB112098) at dilution of 1: 800
	Immunofluorescent analysis of paraformaldehyde-fixed mouse testis using Cyclin B1 (GB112098) at dilution of 1: 800
	Immunofluorescent analysis of paraformaldehyde-fixed rat testis using Cyclin B1 (GB112098) at dilution of 1: 800
	Immunofluorescent analysis of paraformaldehyde-fixed rat colon using Cyclin B1 (GB112098) at dilution of 1: 800

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer .

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