Anti -MCM2 Rabbit pAb

價格：在線咨詢

貨號：

KT012112

產(chǎn)品規(guī)格：

100 μL

Applications：

WB/IHC/IF

Species：

HMR/MR/MR

品牌：

萬物

購買數(shù)量：

立即詢價在線咨詢 400-1016-218

產(chǎn)品詳情操作說明注意事項

Product Information

Description	MCM2 rabbit polyclonal
Protein full name	DNA replication licensing factor MCM2
Synonyms	Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, MCM2, Bm28, Cdcl1, Kiaa0030, Mcmd2
Immunogen	Recombinant protein corresponding to Mouse MCM2
Isotype	IgG
Purity	Affinity purification
Subcellular location	Nucleus
Predicted MW.	102 kDa
Observed MW.	130 kDa
Uniprot ID	P49736, P97310, D3ZP96

Applications

Applications	Species	Dilution	Positive Tissue
WB	Human, Mouse, Rat	1: 500-1: 1000	lymph node, thymus
IHC	Mouse, Rat	1: 500-1: 2000	spleen, small intestine, colon
IF	Mouse, Rat	1: 1800-1: 3600

Background

The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.

Images

	Western blot analysis of MCM2 (GB111361) at dilution of 1: 1000 Lane 1: Hela cell lysate Lane 2: Jurkat cell lysate Lane 3: MCF7 cell lysate Lane 4: K562 cell lysate Lane 5: NIH3T3 cell lysate Lane 6: Mouse lymph node tissue lysate Lane 7: Mouse thymus tissue lysate Lane 8: Rat lymph node tissue lysate Lane 9: Rat thymus tissue lysate
	Immunohistochemistry analysis of paraffin-embedded mouse spleen using MCM2 (GB111361) at dilution of 1: 950
	Immunohistochemistry analysis of paraffin-embedded rat spleen using MCM2 (GB111361) at dilution of 1: 950
	Immunohistochemistry analysis of paraffin-embedded mouse small intestine using MCM2 (GB111361) at dilution of 1: 1000
	Immunohistochemistry analysis of paraffin-embedded rat colon using MCM2 (GB111361) at dilution of 1: 1000

Storage

Storage	Store at -20 ℃ for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.

NOTE:

1.This product is intended for research only.

2.This product is recommended to dilute with the Primary Antibody Dilution Buffer （G2025）.

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